|
KMID : 1812220180010010005
|
|
Journal of The Korean Academy of Sports Dentistry
2018 Volume.1 No. 1 p.5 ~ p.10
|
|
|
PDL cell viability according to concentrations of epigallocatechin-3-gallate
|
|
Yoo Eun-Kyu
Nam Ok-Hyung
Kim Mi-Sun
Lee Hyo-Seol
Kim Kwang-Chul
Choi Sung-Chul
|
|
|
Abstract
|
|
|
Purpose: The aim of this study was to evaluate the effect of epigallocatechin?3?gallate (EGCG) as a storage media on the viability of human periodontal ligament (PDL) cell.
Materials and Methods: Human PDL cells were collected and incubated from the healthy premolars that were extracted for orthodontic treatment. The cultures were exposed at tap water, HBSS, and EGCG (1, 5, 10, 20, 50, and 100 μM) for 24 hrs. The viability of PDL cells was determined using MTS assay and the survival rate was counted by Nucleocounter system.
Results: PDL cells in less than 5 μM EGCG showed a similar rate of cell viability compared to HBSS (p > 0.05). However, PDL cells in 10, 20, and 50 μM EGCG showed lower rates compared to the HBSS. PDL cells in 100 μM EGCG and tap water demonstrated remarkably decreased rate of viability (p < 0.05). Survival rates of PDL cells in less than 20 μM EGCG was similar with HBSS (p > 0.05). Survival rate of PDL cells in 50 μM EGCG was decreased after 3 hr (p < 0.05). Also, survival rates of PDL cells in 100μM EGCG and tap water were significantly low (p < 0.05).
Conclusion: PDL cells stored in less than 20 μM appear to be effective concentration for preserving the viability of PDL cells.
|
|
|
KEYWORD
|
|
|
Epigallocatechin-3-gallate, periodontal ligament cell, avulsion, storage medium
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|
|